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KMID : 0613820040140030522
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Journal of Life Science
2004 Volume.14 No. 3 p.522 ~ p.525
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Genetic Diversity Analysis of the Cheju Horse Using Random Amplified Polymorphic DNAs
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Cho Byung-Wook
Lee Kil-Wang
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Abstract
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This experiment was carried out to analyze genetic characteristics and to develop the breed specific DNA marker for Cheju-native horse. If this marker contains high repetitive sequences, it is possible to convert a RAPD marker of interest into a single-locus PCR marker called a sequence characterized amplified region(SCAR). Twenty six Cheju-native horse and Fifty thoroughbred genomic DNA were pooled and PCR. were accomplished using 800 random primers. Comparing the pooled DNA from Cheju-native horse and thoroughbred, we found 9 primers which identified markers present in the pooled DNA from breed but absent in the other breed. Among 9 random primers, 6 primers were thoroughbred specific and 3 primers were Cheju-native horse specific. Testing individual horse revealed that 5 marker showed the similar band pattern between Cheju-native horse and Thoroughbred. However, 4 marker were wholly absent in breed while present in the other breed. UBC 1263500bp, UBC 162500bp, and UBC 2441200bp was detected only Thoroughbred and UBC 562560bp was detected Cheju-native horse, respectively. After determining of the cloned breed-specific fragment sequence, we designed the SCAR-primers and carried out PCR. Compared to random primer, RAPD-SCAR primer didn’t show significantly higher specific band. However, RAPD analysis is useful for genetic characterization of Cheju-native horse
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KEYWORD
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Cheju-native horse, Thoroughbred, RAPD-PCR, SCAR
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